Pax7 Antibody (PAX7/497)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32894
![Western Blot: Pax7 Antibody (PAX7/497) [NBP2-32894]](https://resources.bio-techne.com/images/products/Pax7-Antibody-PAX7-497-Western-Blot-NBP2-32894-img0003.jpg "Western Blot: Pax7 Antibody (PAX7/497) [NBP2-32894]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Zebrafish
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PAX7/497
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)
Localization
Nuclear
Marker
Rhabdomyosarcoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34706)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Pax7 Antibody (PAX7/497)
Western Blot: Pax7 Antibody (PAX7/497) [NBP2-32894]
Western Blot: Pax7 Antibody (PAX7/497) [NBP2-32894] - Western Blot of HeLa cell lysate using Pax7 Antibody (PAX7/497).Applications for Pax7 Antibody (PAX7/497)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Use in IHC-Fr reported in scientific literature PMID: (32353175).
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Pax7
Additional Pax7 Products
Product Documents for Pax7 Antibody (PAX7/497)
Product Specific Notices for Pax7 Antibody (PAX7/497)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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