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Pax7 Antibody (PAX7/497) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34706

Novus Biologicals, part of Bio-Techne
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NBP2-34706-0.1mg

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Zebrafish

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PAX7/497

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)

Localization

Nuclear

Marker

Rhabdomyosarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32894).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Pax7 Antibody (PAX7/497) - Azide and BSA Free

Western Blot: Pax7 Antibody (PAX7/497)Azide and BSA Free [NBP2-34706]

Western Blot: Pax7 Antibody (PAX7/497)Azide and BSA Free [NBP2-34706]

Western Blot: Pax7 Antibody (PAX7/497) - Azide and BSA Free [NBP2-34706] - analysis of PAX7 in human HeLa Cell lysate

Applications for Pax7 Antibody (PAX7/497) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Frozen

0.5-1ug/ml

Western Blot

0.5-1ug/ml
Application Notes
Use in IHC-Fr reported in scientific literature PMID: (32353175).
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Pax7

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

Long Name

Paired Box Gene 7

Alternate Names

HUP1, RMS2

Gene Symbol

PAX7

Additional Pax7 Products

Product Documents for Pax7 Antibody (PAX7/497) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pax7 Antibody (PAX7/497) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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