PAX8 Antibody (CL7834) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44319

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL7834

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PAX8 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q06710, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PAX8 Antibody (CL7834) - Azide and BSA Free

PAX8 Antibody (CL7834) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP3-44319]

Immunocytochemistry/ Immunofluorescence: PAX8 Antibody [NBP3-44319]

Staining of EFO-21 cells using the Anti-PAX8 monoclonal antibody) .
PAX8 Antibody (CL7834) - Azide and BSA Free Immunohistochemistry-Paraffin: PAX8 Antibody [NBP3-44319]

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP3-44319]

Staining of human Fallopian tube shows moderate to strong nuclear positivity in glandular cells.
PAX8 Antibody (CL7834) - Azide and BSA Free Immunohistochemistry-Paraffin: PAX8 Antibody [NBP3-44319]

Immunohistochemistry-Paraffin: PAX8 Antibody [NBP3-44319]

Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.
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Applications for PAX8 Antibody (CL7834) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PAX8

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described.

Alternate Names

paired box 8, paired box gene 8, paired box protein Pax-8, paired domain gene 8

Gene Symbol

PAX8

Additional PAX8 Products

Product Documents for PAX8 Antibody (CL7834) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PAX8 Antibody (CL7834) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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