PCNA Antibody (PCNA/694) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47835

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PCNA/694

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all PCNA Antibodies »

Product Summary for PCNA Antibody (PCNA/694) - Azide and BSA Free

Immunogen

Recombinant full length human PCNA protein (Uniprot: P12004)

Reactivity Notes

Shows broad species reactivity.

Localization

Predominantly nuclear, some cytoplasmic

Specificity

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This monoclonal antibody is excellent for multiple applications.

Marker

G1- & S-phase Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44731).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for PCNA Antibody (PCNA/694) - Azide and BSA Free

Western Blot: PCNA Antibody (PCNA/694)Azide and BSA Free [NBP2-47835]

Western Blot: PCNA Antibody (PCNA/694)Azide and BSA Free [NBP2-47835]

Western Blot: PCNA Antibody (PCNA/694) - Azide and BSA Free [NBP2-47835] - Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)
Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) - Azide and BSA Free [NBP2-47835]

Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) - Azide and BSA Free [NBP2-47835]

Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) - Azide and BSA Free [NBP2-47835] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)
Flow Cytometry: PCNA Antibody (PCNA/694) - Azide and BSA Free [NBP2-47835]

Flow Cytometry: PCNA Antibody (PCNA/694) - Azide and BSA Free [NBP2-47835]

Flow Cytometry: PCNA Antibody (PCNA/694) - Azide and BSA Free [NBP2-47835] - Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Antibody (PCNA/694) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for PCNA Antibody (PCNA/694) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.25 - 0.5 ug/ml

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: PCNA

Proliferating Cell Nuclear Antigen (PCNA) is a DNA polymerase that is essential for DNA replication, excision and mismatch repair pathways. PCNA is a "DNA clamp" which binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA antibodies are also useful tools for DNA repair and cancer research.

Long Name

Proliferating Cell Nuclear Antigen

Alternate Names

Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbol

PCNA

Additional PCNA Products

Product Documents for PCNA Antibody (PCNA/694) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PCNA Antibody (PCNA/694) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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