PCNA Antibody (PCNA/694) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48284
![Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) - IHC-Prediluted [NBP2-48284]](https://resources.bio-techne.com/images/products/PCNA-Antibody-PCNA-694-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48284-img0001.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) - IHC-Prediluted [NBP2-48284]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # PCNA/694
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PCNA Antibody (PCNA/694) - IHC-Prediluted
Immunogen
Recombinant full length human PCNA protein (Uniprot: P12004)
Reactivity Notes
Shows broad species reactivity.
Localization
Predominantly nuclear, some cytoplasmic
Specificity
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This monoclonal antibody is excellent for multiple applications.
Marker
G1- & S-phase Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for PCNA Antibody (PCNA/694) - IHC-Prediluted
Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) - IHC-Prediluted [NBP2-48284]
Immunohistochemistry-Paraffin: PCNA Antibody (PCNA/694) - IHC-Prediluted [NBP2-48284] - Human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)Applications for PCNA Antibody (PCNA/694) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PCNA
Long Name
Proliferating Cell Nuclear Antigen
Alternate Names
Cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
Gene Symbol
PCNA
Additional PCNA Products
Product Documents for PCNA Antibody (PCNA/694) - IHC-Prediluted
Product Specific Notices for PCNA Antibody (PCNA/694) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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