PDGFR alpha Antibody (16A1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44581

Clone 16A1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, In vitro assay

Cited:

In vitro assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 16A1

Format

BSA Free

Concentration

1 mg/ml

View all PDGF R alpha Antibodies »

Product Specifications

Immunogen

CD140a-transfected NIH 3T3 cells

Specificity

The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PDGFR alpha Antibody (16A1) - BSA Free

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Immunocytochemistry/ Immunofluorescence: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Immunocytochemistry/Immunofluorescence: PDGFR alpha Antibody (16A1) [NBP1-44581] - Characterization of Ad vectors expressing human PDGFRalpha and EGFR. ARPE-19 epithelial, human foreskin fibroblasts (HFF), and human endothelial cells (HUVECs) were transduced with Ad vectors expressing tet-trans, PDGFRalpha, or EGFR as described above, incubated for 24 hr then fixed and stained with the PDGFRalpha-specific antibody AF-307-NA or EGFR-specific antibody LA-1 followed by a FITC-conjugated donkey anti-goat (Molecular Probes) or a Dylight 594-congugated goat anti-mouse secondary antibody. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1002905), licensed under a CC-BY license.
Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow (Cell Surface): PDGFR alpha Antibody (16A1) [NBP1-44581] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.
Flow Cytometry: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow Cytometry: PDGFR alpha Antibody (16A1) - BSA Free [NBP1-44581]

Flow Cytometry: PDGFR alpha Antibody (16A1) [NBP1-44581] - CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.
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Applications for PDGFR alpha Antibody (16A1) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
This antibody is Cytof ready. Use in In vitro reported in scientific literature (PMID: 23028311).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDGF R alpha

CD140a / PDGF-RA (platelet-derived growth factor receptor alpha) is a cell surface receptor for members of platelet-derived growth factor family, whose intracellular part contains a tyrosine kinase domain. CD140a forms homodimers, or heterodimerizes with CD140b / PDGF-RB. Whereas CD140b induces in different cell types their proliferation and migration, the role of CD140a is more controversial, with pro-proliferative or anti-proliferative effects. CD140a has early developmental functions, mediates mesodermal cell migration, and later acts in signaling associated in epithelial-mesenchymal interactions.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (16A1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PDGFR alpha Antibody (16A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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