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PDGFR alpha Antibody (16A1) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47239

Clone 16A1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-47239
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Key Product Details

Species Reactivity

Human

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 16A1

Concentration

1 mg/ml

Product Specifications

Immunogen

CD140a-transfected NIH 3T3 cells

Specificity

The mouse monoclonal antibody 16A1 recognizes CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumours.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Store in the dark. Avoid prolonged exposure to light.

Scientific Data Images for PDGFR alpha Antibody (16A1) [Biotin]

Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) purified / GAM-APC.
Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected 3T3 cells using anti-CD140a (16A1) APC.
Flow (Cell Surface): PDGF R alpha Antibody (16A1) [Biotin] [NBP1-47239] - Surface staining of CD140a in CD140a-transfected cells using anti-CD140a (16A1) PE.

Applications for PDGFR alpha Antibody (16A1) [Biotin]

Application
Recommended Usage

Flow Cytometry

1-2 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PDGF R alpha

The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents for PDGFR alpha Antibody (16A1) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PDGFR alpha Antibody (16A1) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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