PDGFR alpha Antibody (APA5) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43350
![Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]](https://resources.bio-techne.com/images/products/PDGF-R-alpha-Antibody-APA5-Flow-Cytometry-NBP1-43350-img0003.jpg "Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # APA5
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was CD140a.
Reactivity Notes
Human reactivity reported from a verified customer review.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for PDGFR alpha Antibody (APA5) - BSA Free
Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]
Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Analysis using the Biotin conjugate of NBP1-43350. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE . Appropriate isotype controls were used (open histogram).![Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]](https://resources.bio-techne.com/images/products/PDGF-R-alpha-Antibody-APA5-Flow-Cytometry-NBP1-43350-img0001.jpg "Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]")
Flow Cytometry: PDGFR alpha Antibody (APA5) - BSA Free [NBP1-43350]
Flow Cytometry: PDGF R alpha Antibody (APA5) [NBP1-43350] - Staining of NIH/3T3 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE . Total viable cells were used for analysisApplications for PDGFR alpha Antibody (APA5) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8cells/test.
In flow cytometry, we recommend using a cell surface staining protocol and fixation and permeabilization is not required to get staining. If fixation/permeabilization is needed for other markers in the same experiment, we recommend doing all surface staining prior to the fix/perm procedure. This antibody may not bind to a fixed epitope.
In flow cytometry, we recommend using a cell surface staining protocol and fixation and permeabilization is not required to get staining. If fixation/permeabilization is needed for other markers in the same experiment, we recommend doing all surface staining prior to the fix/perm procedure. This antibody may not bind to a fixed epitope.
Reviewed Applications
Read 1 review rated 5 using NBP1-43350 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PDGF R alpha
Long Name
Platelet-derived Growth Factor Receptor alpha
Alternate Names
CD140a, PDGFR alpha, PDGFRA
Entrez Gene IDs
18595 (Mouse)
Gene Symbol
PDGFRA
UniProt
Additional PDGF R alpha Products
Product Documents for PDGFR alpha Antibody (APA5) - BSA Free
Product Specific Notices for PDGFR alpha Antibody (APA5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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