PDGFR alpha Antibody (APA5) [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43581
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry
Label
Biotin
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # APA5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen for this antibody was CD140a.
Reactivity Notes
Human reactivity reported from a verified customer review.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Scientific Data Images for PDGFR alpha Antibody (APA5) [Biotin]
Flow Cytometry: PDGF R alpha Antibody (APA5) [Biotin] [NBP1-43581] - Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140a (PDGF Receptor a) Biotin followed by Streptavidin PE . Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Applications for PDGFR alpha Antibody (APA5) [Biotin]
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Application Notes
The APA5 antibody has been tested by flow cytometric analysis of the NIH-3T3 cell line. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
In flow cytometry, we recommend using a cell surface staining protocol and fixation and permeabilization is not required to get staining. If fixation/permeabilization is needed for other markers in the same experiment, we recommend doing all surface staining prior to the fix/perm procedure. This antibody may not bind to a fixed epitope.
In flow cytometry, we recommend using a cell surface staining protocol and fixation and permeabilization is not required to get staining. If fixation/permeabilization is needed for other markers in the same experiment, we recommend doing all surface staining prior to the fix/perm procedure. This antibody may not bind to a fixed epitope.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: PDGF R alpha
Long Name
Platelet-derived Growth Factor Receptor alpha
Alternate Names
CD140a, PDGFR alpha, PDGFRA
Gene Symbol
PDGFRA
Additional PDGF R alpha Products
Product Documents for PDGFR alpha Antibody (APA5) [Biotin]
Product Specific Notices for PDGFR alpha Antibody (APA5) [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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