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Pepsin Antibody [HRP]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-20732

Novus Biologicals, part of Bio-Techne
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NB120-20732-0.1mg

Key Product Details

Species Reactivity

Validated:

Porcine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

HRP

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Summary for Pepsin Antibody [HRP]

Immunogen

Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791)

Reactivity Notes

Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]

Scientific Data Images for Pepsin Antibody [HRP]

Western Blot: Pepsin Antibody [HRP] [NB120-20732] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin.
Pepsin Antibody [HRP]

Pepsin Antibody [HRP]

Western Blot of Peroxidase conjugated Goat anti-Pepsin antibody. Lane 1: Pepsin. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for 30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Other band(s): none.

Applications for Pepsin Antibody [HRP]

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunohistochemistry

1:250-1:1250

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:2500
Application Notes
This product has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Pepsin

Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.

Alternate Names

DKFZp666J2410, ec 3.4.23.1, Pepsin a precursor, Pepsinogen 1 second locus, Pepsinogen 3 group 1, Pepsinogen 4 group I, Pepsinogen 5 group I, Pepsinogen A, Pepsinogen a3, Pepsinogen A4, Pepsinogen A5, Pepsinogen group 1, Pepsinogen I, Pga 3, Pga 4, Pga 5, pga3, pga4, pga5

Additional Pepsin Products

Product Documents for Pepsin Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pepsin Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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