Pepsin Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-614
Key Product Details
Species Reactivity
Validated:
Porcine
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Summary for Pepsin Antibody [Biotin]
Immunogen
Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791)
Reactivity Notes
Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]
Scientific Data Images for Pepsin Antibody [Biotin]
Western Blot: Pepsin Antibody [Biotin] [NB600-614] - Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin.
Pepsin Antibody [Biotin]
Western Blot of Goat anti-Pepsin Antibody Biotin Conjugated. Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody [Biotin]1:1,000 overnight at 4C. Secondary antibody: HRP StreptavidinApplications for Pepsin Antibody [Biotin]
Application
Recommended Usage
ELISA
1:1000-1:10000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
This product has been tested by ELISA and western. This product is suitable for Immunohistochemistry. Expect band at 41.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Pepsin
Alternate Names
DKFZp666J2410, ec 3.4.23.1, Pepsin a precursor, Pepsinogen 1 second locus, Pepsinogen 3 group 1, Pepsinogen 4 group I, Pepsinogen 5 group I, Pepsinogen A, Pepsinogen a3, Pepsinogen A4, Pepsinogen A5, Pepsinogen group 1, Pepsinogen I, Pga 3, Pga 4, Pga 5, pga3, pga4, pga5
UniProt
Additional Pepsin Products
Product Documents for Pepsin Antibody [Biotin]
Product Specific Notices for Pepsin Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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