Pepsin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44230
Key Product Details
Species Reactivity
Validated:
Porcine
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Summary for Pepsin Antibody
Immunogen
Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791)
Reactivity Notes
Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Pepsin Antibody
Western Blot: Pepsin Antibody [NBP2-44230]
Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230]
Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230] - Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).Western Blot: Pepsin Antibody [NBP2-44230]
Western Blot: Pepsin Antibody [NBP2-44230] - lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane.Applications for Pepsin Antibody
Application
Recommended Usage
ELISA
1:50:000-1:200000
Immunohistochemistry
1:2000-1:10000
Immunoprecipitation
1:100
Western Blot
1:5000-1:20000
Application Notes
This product has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Pepsin
Alternate Names
DKFZp666J2410, ec 3.4.23.1, Pepsin a precursor, Pepsinogen 1 second locus, Pepsinogen 3 group 1, Pepsinogen 4 group I, Pepsinogen 5 group I, Pepsinogen A, Pepsinogen a3, Pepsinogen A4, Pepsinogen A5, Pepsinogen group 1, Pepsinogen I, Pga 3, Pga 4, Pga 5, pga3, pga4, pga5
UniProt
Additional Pepsin Products
Product Documents for Pepsin Antibody
Product Specific Notices for Pepsin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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