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Pepsin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44230

Novus Biologicals, part of Bio-Techne
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NBP2-44230

Key Product Details

Species Reactivity

Validated:

Porcine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

LYOPH mg/ml

Product Summary for Pepsin Antibody

Immunogen

Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791)

Reactivity Notes

Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Pepsin Antibody

Western Blot: Pepsin Antibody [NBP2-44230]

Western Blot: Pepsin Antibody [NBP2-44230]

Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.
Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230]

Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230]

Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230] - Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).
Western Blot: Pepsin Antibody [NBP2-44230]

Western Blot: Pepsin Antibody [NBP2-44230]

Western Blot: Pepsin Antibody [NBP2-44230] - lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane.

Applications for Pepsin Antibody

Application
Recommended Usage

ELISA

1:50:000-1:200000

Immunohistochemistry

1:2000-1:10000

Immunoprecipitation

1:100

Western Blot

1:5000-1:20000
Application Notes
This product has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Pepsin

Digestive enzyme with particularly broad specificity.

Alternate Names

DKFZp666J2410, ec 3.4.23.1, Pepsin a precursor, Pepsinogen 1 second locus, Pepsinogen 3 group 1, Pepsinogen 4 group I, Pepsinogen 5 group I, Pepsinogen A, Pepsinogen a3, Pepsinogen A4, Pepsinogen A5, Pepsinogen group 1, Pepsinogen I, Pga 3, Pga 4, Pga 5, pga3, pga4, pga5

Additional Pepsin Products

Product Documents for Pepsin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pepsin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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