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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Janelia Fluor 585

Antibody Source

Monoclonal Rat IgG2A Clone # CB5.4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Perforin Antibody (CB5.4) [Janelia Fluor® 585]

Immunogen

Recombinant mouse perforin (aa 98-534).

Specificity

Recognizes mouse perforin (region aa 402-534).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Perforin Antibody (CB5.4) [Janelia Fluor® 585]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Perforin

Perforin (PRF1) is a 70 kD cytolytic protein that is the major protein constituent of cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Specifically, perforin enters the target cell's plasma membrand and creates a intercellular pore to mediate targeted cell lysis. PRF1 antibodies are useful tools for lysis research on both CTLs and NK cells.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Gene Symbol

PRF1

Additional Perforin Products

Product Documents for Perforin Antibody (CB5.4) [Janelia Fluor® 585]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Perforin Antibody (CB5.4) [Janelia Fluor® 585]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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