Perforin Antibody (CE2.10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97440
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CE2.10
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for Perforin Antibody (CE2.10) - BSA Free
Immunogen
Recombinant mouse perforin (aa 98-534).
Reactivity Notes
Immunoprecipitation (Human and Mouse), Western Blot (Mouse only). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
Recognizes an epitope in the region aa 402-534 of human and mouse perforin. Does NOT detect Human in Western Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Perforin Antibody (CE2.10) - BSA Free
Western Blot: Perforin Antibody (CE2.10)BSA Free [NBP1-97440]
Western Blot: Perforin Antibody (CE2.10) [NBP1-97440] - Detectioon of perforin in the mouse T cell clone B6.1. (lane 2). As control cell line HEK 293 was used (lane 1). The protein migrates around ~66kDa.Method: Cell extracts from the T cell clone B6.1 (2x10^6) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10. antibody at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.Applications for Perforin Antibody (CE2.10) - BSA Free
Application
Recommended Usage
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
This antibody is useful in Immunoprecipitation (Human and Mouse) and Western Blot (Mouse ONLY).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Perforin
Long Name
Perforin 1 (Pore Forming Protein)
Alternate Names
Cytolysin, FLH2, HPLH2, P1, PFP, PRF1
Gene Symbol
PRF1
Additional Perforin Products
Product Documents for Perforin Antibody (CE2.10) - BSA Free
Product Specific Notices for Perforin Antibody (CE2.10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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