Perforin Antibody (CE2.10) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97440CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2A Clone # CE2.10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Perforin Antibody (CE2.10) [CoraFluor™ 1]
Immunogen
Recombinant mouse perforin (aa 98-534).
Reactivity Notes
Immunoprecipitation (Human and Mouse), Western Blot (Mouse only). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
Recognizes an epitope in the region aa 402-534 of human and mouse perforin. Does NOT detect Human in Western Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Perforin Antibody (CE2.10) [CoraFluor™ 1]
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Perforin
Long Name
Perforin 1 (Pore Forming Protein)
Alternate Names
Cytolysin, FLH2, HPLH2, P1, PFP, PRF1
Gene Symbol
PRF1
Additional Perforin Products
Product Documents for Perforin Antibody (CE2.10) [CoraFluor™ 1]
Product Specific Notices for Perforin Antibody (CE2.10) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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