Periostin/OSF-2 Antibody (OTI1A1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47922
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # OTI1A1
Concentration
1 mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-230 of human Periostin/OSF-2 (NP_006466) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This antibody will possibly detect all isoforms encoding the amino acid regions 1-230 of human Periostin/OSF-2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Theoretical MW
93.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Periostin/OSF-2 Antibody (OTI1A1)
Western Blot: Periostin/OSF-2 Antibody (OTI1A1) [NBP1-47922]
Western Blot: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human Periostin gene expression vector.Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (OTI1A1) [NBP1-47922]
Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody (1A1) [NBP1-47922] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY POSTN.Applications for Periostin/OSF-2 Antibody (OTI1A1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
Reactivity reported in (PMID:22512703).
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Periostin/OSF-2
Long Name
Osteoblast Specific Factor 2
Alternate Names
Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52
Entrez Gene IDs
10631 (Human)
Gene Symbol
POSTN
UniProt
Additional Periostin/OSF-2 Products
Product Documents for Periostin/OSF-2 Antibody (OTI1A1)
Product Specific Notices for Periostin/OSF-2 Antibody (OTI1A1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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