Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73335

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # OTI1A1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Periostin/OSF-2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-230 of human periostin (NP_006466) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

93.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free

Western Blot: Periostin/OSF-2 Antibody (OTI1A1)Azide and BSA Free [NBP2-73335]

Western Blot: Periostin/OSF-2 Antibody (OTI1A1)Azide and BSA Free [NBP2-73335]

Western Blot: Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-73335] - Periostin antibody ( 47 ) at 1:500 dilution + lysate from HeLa cells transfected with human Periostin gene expression vector.
Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-73335]

Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-73335]

Immunocytochemistry/Immunofluorescence: Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free [NBP2-73335] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY POSTN.

Applications for Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Periostin/OSF-2

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Long Name

Osteoblast Specific Factor 2

Alternate Names

Fasciclin I-like, OSF-2, OSF2, POSTN, TRIF52

Gene Symbol

POSTN

Additional Periostin/OSF-2 Products

Product Documents for Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Periostin/OSF-2 Antibody (OTI1A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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