PHLPP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1812

Novus Biologicals, part of Bio-Techne
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NB100-1812
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PHLPP2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1275 and the C-terminus (residue 1323) of human PH Domain and Leucine Rich Repeat Protein Phosphatase-like using the numbering given in entry NP_055835.2 (GeneID 23035).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23993956).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PHLPP2 Antibody

Western Blot: PHLPP2 Antibody [NB100-1812]

Western Blot: PHLPP2 Antibody [NB100-1812]

Western Blot: PHLPP2 Antibody [NB100-1812] - Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PHLPPL antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
PHLPP2 Antibody [NB100-1812] - Detection of human PHLPPL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-PHLPPL antibody NB100-1812 (lot NB100-1812-2) used for IP at 6 ug per reaction. PHLPPL was also immunoprecipitated by a previous lot of this antibody (lot NB100-1812-1). For blotting immunoprecipitated PHLPPL, NB100-1812 was used at 0.04 ug/ml. Chemiluminescence with an exposure time of 10 seconds.

Applications for PHLPP2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/ml

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PHLPP2

PHLPPL (PH domain leucine-rich repeat-containing protein phosphatase-like) is responsible for the dephosphorylation and subsequent inactivation of Akt. Akt is a signaling protein critical to the balance of cell survival and apoptosis. Dephosphorylation of Akt by PHLPPL has been demonstrated to trigger apoptosis and suppress tumor cell growth. PHLPPL is also known as PHLPPL2 and functions similarly to its relative, PHLPP1. Recent studies show that PHLPPL and PHLPP selectively terminate Akt-signaling by targeting specific Akt isoforms. Alternate names for PHLPPL include PH Domain and Leucine Rich Repeat Protein Phosphatase-Like, KIAA0931, and PHLPP2.

Alternate Names

EC 3.1.3.16, KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase-like, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPPLPHLPP-like

Entrez Gene IDs

23035 (Human); 244650 (Mouse)

Gene Symbol

PHLPP2

UniProt

Q6ZVD8 (Human)

Additional PHLPP2 Products

Product Documents for PHLPP2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PHLPP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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