PHLPP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61668

Novus Biologicals, part of Bio-Techne
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NB100-61668
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PHLPP2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-PHEEDRTEPPEEFD corresponding to C-Terminus according to NP_055835.2.

Epitope

PHEEDRTEPPEEFD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PHLPP2 Antibody

Western Blot: PHLPP2 Antibody [NB100-61668]

Western Blot: PHLPP2 Antibody [NB100-61668]

Western Blot: PHLPP2 Antibody [NB100-61668] - Staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PHLPP2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 140 kDa band observed in human brain (amygdala, hippocampus, substantia nigra) lysates (calculated MW of 134 kDa band according to NP_055835.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PHLPP2

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Alternate Names

EC 3.1.3.16, KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase-like, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPPLPHLPP-like

Entrez Gene IDs

23035 (Human)

Gene Symbol

PHLPP2

UniProt

NP_055835.2 (Human)

Additional PHLPP2 Products

Product Documents for PHLPP2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHLPP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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