Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PHLPP2 Antibodies »

Product Specifications

Immunogen

PHLPP2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human PHLPP2. The immunogen is located within the last 50 amino acids of PHLPP2. Amino Acid Squence: DSRLEPEPHEEDRTEPPEEF

Specificity

At least three isoforms are known to exist; this antibody will detect the two largest isoforms. PHLPP2 antibody is predicted to not cross react with PHLPP1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

156 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PHLPP2 Antibody - BSA Free

Western Blot: PHLPP2 AntibodyBSA Free [NBP2-81874]

Western Blot: PHLPP2 AntibodyBSA Free [NBP2-81874]

Western Blot: PHLPP2 Antibody [NBP2-81874] - Analysis of PHLPP2 in SW480 cell lysate with PHLPP2 antibody at 1 ug/ml in (A) the presence and (B) absence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: PHLPP2 Antibody - BSA Free [NBP2-81874]

Immunocytochemistry/ Immunofluorescence: PHLPP2 Antibody - BSA Free [NBP2-81874]

Immunocytochemistry/Immunofluorescence: PHLPP2 Antibody [NBP2-81874] - Immunofluorescence of PHLPP2 in SW480 cells with PHLPP2 antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: PHLPP2 Antibody - BSA Free [NBP2-81874]

Immunocytochemistry/ Immunofluorescence: PHLPP2 Antibody - BSA Free [NBP2-81874]

Immunocytochemistry/Immunofluorescence: PHLPP2 Antibody [NBP2-81874] - Immunocytochemistry of PHLPP2 in SW480 cells with PHLPP2 antibody at 2.5 ug/ml.
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Applications for PHLPP2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PHLPP2

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Alternate Names

EC 3.1.3.16, KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase 2, PH domain and leucine rich repeat protein phosphatase-like, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPPLPHLPP-like

Gene Symbol

PHLPP2

Additional PHLPP2 Products

Product Documents for PHLPP2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PHLPP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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