Phospho-Tyrosine Antibody (RM111)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77400

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-77400
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

All Species

Applications

Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM111

Concentration

1 mg/ml

View all Phospho-Tyrosine Antibodies »

Product Summary for Phospho-Tyrosine Antibody (RM111)

Immunogen

Phospho-Tyrosine -BSA conjugate

Specificity

This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non-phosphorylated tyrosine, phosphoserine, and phosphorthreonine

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-Tyrosine Antibody (RM111)

Western Blot: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]

Western Blot: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]

Western Blot: Phospho-Tyrosine Antibody (RM111) [NBP2-77400] - Western blot of serum-starved A431 cells nontreated or treated with EGF, using NBP2-77400. The blot was exposed to the film at different time points.
Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]

Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]

Immunocytochemistry/Immunofluorescence: Phospho-Tyrosine Antibody (RM111) [NBP2-77400] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using NBP2-77400 (followed by a PE conjugated secondary antibody, red) and DAPI (blue).
Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]

Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) [NBP2-77400]

Multiplex Immunoassay: Phospho-Tyrosine Antibody (RM111) [NBP2-77400] - Luminex analysis of NBP2-77400's reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. NBP2-77400 reacts only to phosphorylated Tyrosine.
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Applications for Phospho-Tyrosine Antibody (RM111)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:200-1:1000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:500

Immunohistochemistry

1:200 - 1:500

Immunoprecipitation

1:200-1:1000

Western Blot

1:1000 -1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phospho-Tyrosine

Phosphotyrosine is the phosphorylated version of the amino acid tyrosine. Phosphorylation of histidine, serine, threonine and tyrosine residues acts as a signalling system to control many cellular signalling pathways. The cellular networks which rely on these signals can be very complex, so phosphorylation often occurs on multiple sites on a given protein.

Alternate Names

PhosphoTyrosine

Additional Phospho-Tyrosine Products

Product Documents for Phospho-Tyrosine Antibody (RM111)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phospho-Tyrosine Antibody (RM111)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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