Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25981
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
All Species
Applications
Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM111
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Phospho-Tyrosine -BSA conjugate
Specificity
This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non-phosphorylated tyrosine, phosphoserine, and phosphorthreonine
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free
Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] -
Immunoprecipitation: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunoprecipitation of EGF-treated A431 cells by Clone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by Clone RM111.
Western Blot: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] -
Western Blot: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Western blot of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/5000 dilution. The blot was exposed to the film at different time points.
Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] -
Immunocytochemistry/ Immunofluorescence: Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free [NBP3-25981] - Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/500 dilution followed by a PE conjugated secondary antibody (red) and DAPI (blue).Applications for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:200-1:1000
Flow Cytometry
1:10 - 1:1000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:500
Immunohistochemistry
1:200 - 1:500
Immunoprecipitation
1:200-1:1000
Western Blot
1:1000 -1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Phospho-Tyrosine
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free
Product Specific Notices for Phospho-Tyrosine Antibody (RM111) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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