PKN2 Antibody (20A8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26379
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 20A8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human PKN2 [UniProt Q16513]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PKN2 Antibody (20A8)
Immunohistochemistry: PKN2 Antibody (20A8) [NBP3-26379] -
Immunohistochemistry: PKN2 Antibody (20A8) [NBP3-26379] - Image of PKN2 Antibody (20A8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: PKN2 Antibody (20A8) [NBP3-26379] -
Western Blot: PKN2 Antibody (20A8) [NBP3-26379] - Positive Western Blot detected in: Hela whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate.All lanes: PKN2 Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 113, 117, 112 kDa
Observed band size: 130 kDa
Immunocytochemistry/Immunofluorescence: PKN2 Antibody (20A8) [NBP3-26379] -
Immunocytochemistry/Immunofluorescence: PKN2 Antibody (20A8) [NBP3-26379] - Staining of MCF-7 cell with PKN2 Antibody (20A8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 509-conjugated Goat Anti-Rabbit IgG (H+L).Applications for PKN2 Antibody (20A8)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PKN2
Long Name
Protein Kinase N2
Alternate Names
PAK2, PRK2, PRKCL2, PRO2042
Gene Symbol
PKN2
Additional PKN2 Products
Product Documents for PKN2 Antibody (20A8)
Product Specific Notices for PKN2 Antibody (20A8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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