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PKN2 Antibody (3A7)

Novus Biologicals, part of Bio-Techne | Catalog # H00005586-M01

Novus Biologicals, part of Bio-Techne
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H00005586-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3A7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PKN2 (NP_006247, 580 a.a. ~ 679 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNT

Specificity

PKN2 - protein kinase N2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKN2 Antibody (3A7)

Immunocytochemistry/ Immunofluorescence: PKN2 Antibody (3A7) [H00005586-M01]

Immunocytochemistry/ Immunofluorescence: PKN2 Antibody (3A7) [H00005586-M01]

Immunocytochemistry/Immunofluorescence: PKN2 Antibody (3A7) [H00005586-M01] - Analysis of monoclonal antibody to PKN2 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: PKN2 Antibody (3A7) [H00005586-M01] - Detection limit for recombinant GST tagged PKN2 is approximately 0.1ng/ml as a capture antibody.

Applications for PKN2 Antibody (3A7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKN2

Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis

Long Name

Protein Kinase N2

Alternate Names

PAK2, PRK2, PRKCL2, PRO2042

Entrez Gene IDs

5586 (Human)

Gene Symbol

PKN2

OMIM

602549 (Human)

Additional PKN2 Products

Product Documents for PKN2 Antibody (3A7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKN2 Antibody (3A7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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