PKN2 Antibody (5O2G6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15891

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15891-100ul
NBP3-15891-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5O2G6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKN2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PKN2 (Q16513). MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLRKVTTDKKSLAYVDNILKKSNKKLEELHHKL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKN2 Antibody (5O2G6)

Western Blot: PKN2 Antibody (5O2G6) [NBP3-15891]

Western Blot: PKN2 Antibody (5O2G6) [NBP3-15891]

Western Blot: PKN2 Antibody (5O2G6) [NBP3-15891] - Western blot analysis of extracts of various cell lines, using PRK2/PKN2 Rabbit mAb (NBP3-15891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for PKN2 Antibody (5O2G6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKN2

Protein-kinase-C-related kinases (PRKs) (1) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN (2-3). Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis (4).

Long Name

Protein Kinase N2

Alternate Names

PAK2, PRK2, PRKCL2, PRO2042

Gene Symbol

PKN2

Additional PKN2 Products

Product Documents for PKN2 Antibody (5O2G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKN2 Antibody (5O2G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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