PKN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31367

Novus Biologicals, part of Bio-Techne
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NBP1-31367
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (96%), Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKN2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKN2. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKN2 Antibody

Western Blot: PKN2 Antibody [NBP1-31367]

Western Blot: PKN2 Antibody [NBP1-31367]

Western Blot: PKN2 Antibody [NBP1-31367] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PKN2 Antibody [NBP1-31367]

Immunocytochemistry/ Immunofluorescence: PKN2 Antibody [NBP1-31367]

Immunocytochemistry/Immunofluorescence: PKN2 Antibody [NBP1-31367] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
PKN2 Antibody

Immunohistochemistry: PKN2 Antibody [NBP1-31367] -

Immunohistochemistry: PKN2 Antibody [NBP1-31367] - PRK2 antibody [N1N3] detects PRK2 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
PRK2 antibody [N1N3] (NBP1-31367) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Applications for PKN2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKN2

Protein-kinase-C-related kinases (PRKs) (1) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN (2-3). Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis (4).

Long Name

Protein Kinase N2

Alternate Names

PAK2, PRK2, PRKCL2, PRO2042

Gene Symbol

PKN2

Additional PKN2 Products

Product Documents for PKN2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PKN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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