PKN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68736

Novus Biologicals, part of Bio-Techne
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NBP2-68736
NBP2-68736-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKN2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKN2 Antibody

Western Blot: PKN2 Antibody [NBP2-68736]

Western Blot: PKN2 Antibody [NBP2-68736]

Western Blot: PKN2 Antibody [NBP2-68736] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: PKN2 Antibody [NBP2-68736]

Immunocytochemistry/ Immunofluorescence: PKN2 Antibody [NBP2-68736]

Immunocytochemistry/Immunofluorescence: PKN2 Antibody [NBP2-68736] - Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunocytochemistry/ Immunofluorescence: PKN2 Antibody [NBP2-68736]

Immunocytochemistry/ Immunofluorescence: PKN2 Antibody [NBP2-68736]

Immunocytochemistry/Immunofluorescence: PKN2 Antibody [NBP2-68736] - Staining of human cell line U-2 OS shows localization to nuclear bodies, cytosol & intermediate filaments.

Applications for PKN2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
Recommended conditions for ICC/IF: Fixation/Permeabilization: PFA/Triton X-100, Recommended conditions for IHC: Retrieval method: HIER pH6

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKN2

Protein-kinase-C-related kinases (PRKs) (1) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN (2-3). Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis (4).

Long Name

Protein Kinase N2

Alternate Names

PAK2, PRK2, PRKCL2, PRO2042

Gene Symbol

PKN2

Additional PKN2 Products

Product Documents for PKN2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PKN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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