PLA2R1 Antibody (CL0474) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43909

Novus Biologicals, part of Bio-Techne
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NBP3-43909
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BSA Free
NBP2-52933
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0474

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PLA2R1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q13018, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PLA2R1 Antibody (CL0474) - Azide and BSA Free

PLA2R1 Antibody (CL0474) - Azide and BSA Free Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP3-43909]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP3-43909]

Analysis in human kidney and pancreas tissues using NBP3-43909 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
PLA2R1 Antibody (CL0474) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody [NBP3-43909]

Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody [NBP3-43909]

Staining of HeLa cells using the Anti-PLA2R1 monoclonal antibody) .
PLA2R1 Antibody (CL0474) - Azide and BSA Free Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP3-43909]

Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP3-43909]

Staining of human kidney shows weak membranous positivity in cells in glomeruli.
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Applications for PLA2R1 Antibody (CL0474) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:20000 - 1:50000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLA2R1

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Gene Symbol

PLA2R1

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody (CL0474) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLA2R1 Antibody (CL0474) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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