PLC-beta 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38220

Novus Biologicals, part of Bio-Techne
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NBP2-38220-25ul
NBP2-38220
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Rat

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLC-beta 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NKKKSHKSSEGSGKKKLSEQASNTYSDSSSMFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLC-beta 1 Antibody

Western Blot: PLC-beta 1 Antibody [NBP2-38220]

Western Blot: PLC-beta 1 Antibody [NBP2-38220]

Western Blot: PLC-beta 1 Antibody [NBP2-38220] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma
Immunohistochemistry-Paraffin: PLC-beta 1 Antibody [NBP2-38220]

Immunohistochemistry-Paraffin: PLC-beta 1 Antibody [NBP2-38220]

Immunohistochemistry-Paraffin: PLC-beta 1 Antibody [NBP2-38220] - Staining in human cerebral cortex and pancreas tissues using anti-PLCB1 antibody. Corresponding PLCB1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PLC-beta 1 Antibody [NBP2-38220]

Immunohistochemistry-Paraffin: PLC-beta 1 Antibody [NBP2-38220]

Immunohistochemistry-Paraffin: PLC-beta 1 Antibody [NBP2-38220] - Staining of human cerebral cortex shows high expression.
View 1 more images

Applications for PLC-beta 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-beta 1

Phospholipase C beta 1 is encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Phospholipase C beta 1

Alternate Names

PI-PLC, PLC-154, PLC-I, PLCB1, PLCbeta 1

Gene Symbol

PLCB1

UniProt

Q9NQ66

Additional PLC-beta 1 Products

Product Documents for PLC-beta 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PLC-beta 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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