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Porcine IL-12/IL-23 p40 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9121

R&D Systems, part of Bio-Techne
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MAB9121
MAB9121-SP

Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Porcine

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 116224

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p70 heterodimer

Specificity

Detects the p40 subunit of porcine IL-12/IL-23 in ELISAs and Western blots. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human IL‑12 and IL-23 and no cross-reactivity with recombinant mouse IL-12 or IL-23.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Porcine IL-12/IL-23 p40 Antibody

Application
Recommended Usage

Porcine IL-12 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Porcine IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog # BAM9122)
  • Standard: Recombinant Porcine IL-12 Protein (Catalog # 912-PL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

IL-12 and IL-23 are disulfide-linked heterodimers consisting of a p40 subunit that is common to both cytokines and a unique subunit, p19 in IL-23 or p35 in IL-12.

Long Name

Interleukin 12/Interleukin 23 p40

Alternate Names

CLMF2, IL-12B, IL12 p40, IL12B, NKSF2

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Gene Symbol

IL12B

Additional IL-12/IL-23 p40 Products

Product Documents for Porcine IL-12/IL-23 p40 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Porcine IL-12/IL-23 p40 Antibody

For research use only

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