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Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAM9122

R&D Systems, part of Bio-Techne
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BAM9122

Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Porcine

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development (Detection)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2B Clone # 116211

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p70 heterodimer

Specificity

Detects the p40 subunit of porcine IL-12 and IL-23. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human (rh) IL‑12 and rhIL‑23 and no cross-reactivity with rmIL-12 or rmIL-23.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

Porcine IL-12 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Porcine IL-12/IL-23 p40 Antibody (Catalog # MAB9121)
  • Standard: Recombinant Porcine IL-12 Protein (Catalog # 912-PL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-23 p40

IL-12 and IL-23 are disulfide-linked heterodimers consisting of a p40 subunit that is common to both cytokines and a unique subunit, p19 in IL-23 or p35 in IL-12.

Long Name

Interleukin 12/Interleukin 23 p40

Alternate Names

CLMF2, IL-12B, IL12 p40, IL12B, NKSF2

Entrez Gene IDs

3593 (Human); 16160 (Mouse); 64546 (Rat); 397076 (Porcine); 403976 (Canine); 102124858 (Cynomolgus Monkey); 493741 (Feline); 694747 (Rhesus Macaque)

Gene Symbol

IL12B

Additional IL-12/IL-23 p40 Products

Product Documents for Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Porcine IL‑12/IL‑23 p40 Biotinylated Antibody

For research use only

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