PPM1D Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81881

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PPM1D Antibodies »

Product Specifications

Immunogen

PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D. The immunogen is located within the last 50 amino acids of PPM1D. Amino Acid Squence: KLTMRRRLRGQKKIGNPL

Specificity

PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PPM1D Antibody - BSA Free

Western Blot: PPM1D AntibodyBSA Free [NBP2-81881]

Western Blot: PPM1D AntibodyBSA Free [NBP2-81881]

Western Blot: PPM1D Antibody [NBP2-81881] - Analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: PPM1D Antibody - BSA Free [NBP2-81881]

Immunocytochemistry/ Immunofluorescence: PPM1D Antibody - BSA Free [NBP2-81881]

Immunocytochemistry/Immunofluorescence: PPM1D Antibody [NBP2-81881] - Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 ug/ml.
Immunohistochemistry: PPM1D Antibody - BSA Free [NBP2-81881]

Immunohistochemistry: PPM1D Antibody - BSA Free [NBP2-81881]

Immunohistochemistry: PPM1D Antibody [NBP2-81881] - Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 ug/ml.
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Applications for PPM1D Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPM1D

PPM1D is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Long Name

Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D

Alternate Names

PP2C-delta, Wip1

Gene Symbol

PPM1D

Additional PPM1D Products

Product Documents for PPM1D Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PPM1D Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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