PPM1D Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81881CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-81881AF350

Alexa Fluor 405

NBP2-81881AF405

Alexa Fluor 488

NBP2-81881AF488

Alexa Fluor 532

NBP2-81881AF532

Alexa Fluor 594

NBP2-81881AF594

Alexa Fluor 647

NBP2-81881AF647

Alexa Fluor 700

NBP2-81881AF700

Alexa Fluor 750

NBP2-81881AF750

Biotin

NBP2-81881B

DyLight 350

NBP2-81881UV

DyLight 405

NBP2-81881V

DyLight 488

NBP2-81881G

DyLight 550

NBP2-81881R

DyLight 594

NBP2-81881DL594

DyLight 650

NBP2-81881C

DyLight 680

NBP2-81881FR

DyLight 755

NBP2-81881IR

FITC

NBP2-81881F

HRP

NBP2-81881H

Janelia Fluor 525

NBP2-81881JF525

Janelia Fluor 549

NBP2-81881JF549

Janelia Fluor 585

NBP2-81881JF585

Janelia Fluor 635

NBP2-81881JF635

Janelia Fluor 646

NBP2-81881JF646

Janelia Fluor 669

NBP2-81881JF669

mFluor Violet 450 SE

NBP2-81881MFV450

mFluor Violet 500 SE

NBP2-81881MFV500

mFluor Violet 610 SE

NBP2-81881MFV610

PerCP

NBP2-81881PCP

Forumulation

Catalog #

BSA Free

NBP2-81881

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PPM1D Antibodies »

Product Specifications

Immunogen

PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D. The immunogen is located within the last 50 amino acids of PPM1D. Amino Acid Squence: KLTMRRRLRGQKKIGNPL

Specificity

PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for PPM1D Antibody [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: PPM1D

PPM1D is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Long Name

Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D

Alternate Names

PP2C-delta, Wip1

Gene Symbol

PPM1D

Additional PPM1D Products

Product Documents for PPM1D Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for PPM1D Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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