PRAME Antibody (PRAME/9189)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26784
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PRAME/9189
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa100-250) of human PRAME protein (exact sequence is proprietary)
Localization
Nucleus, Chromosome, Cytoplasm, Golgi apparatus, Cell membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for PRAME Antibody (PRAME/9189)
Immunohistochemistry-Paraffin: PRAME Antibody (PRAME/9189) [NBP3-26784 ] -
Formalin-fixed, paraffin-embedded human melanoma stained with PRAME Antibody (PRAME/9189). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.Applications for PRAME Antibody (PRAME/9189)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml for 30 minutes at RT
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PRAME
Alternate Names
cancer/testis antigen 130, MAPEOIP4CT130, melanoma antigen preferentially expressed in tumors, OIP-4, Opa-interacting protein 4, Opa-interacting protein OIP4, preferentially expressed antigen in melanoma, Preferentially expressed antigen of melanoma
Gene Symbol
PRAME
Additional PRAME Products
Product Documents for PRAME Antibody (PRAME/9189)
Product Specific Notices for PRAME Antibody (PRAME/9189)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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