Prohibitin Antibody (3F4-2B2)

Novus Biologicals, part of Bio-Techne | Catalog # H00005245-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F4-2B2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Prohibitin Antibodies »

Product Specifications

Immunogen

PHB (AAH13401, 1 a.a. ~ 272 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVVFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAGDIAYQLSRSRNITYLPAGQSVLLQLPQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

PHB - prohibitin

Marker

Mitochondria Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Prohibitin Antibody (3F4-2B2)

Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Western Blot: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - PHB monoclonal antibody (M01), clone 3F4-2B2. Analysis of PHB expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Immunocytochemistry/ Immunofluorescence: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Immunocytochemistry/Immunofluorescence: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on HeLa cell. Antibody concentration 1 ~ 10 ug/ml.
Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01]

Immunohistochemistry-Paraffin: Prohibitin Antibody (3F4-2B2) [H00005245-M01] - Analysis of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.Antibody concentration 5 ug/ml.
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Applications for Prohibitin Antibody (3F4-2B2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Prohibitin

Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.

Alternate Names

PHB

Entrez Gene IDs

5245 (Human)

Gene Symbol

PHB

OMIM

176705 (Human)

UniProt

AAH13401 (Human)

Additional Prohibitin Products

Product Documents for Prohibitin Antibody (3F4-2B2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Prohibitin Antibody (3F4-2B2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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