Prohibitin Antibody (PHB/3229)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07602
![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3229-Immunohistochemistry-Paraffin-NBP3-07602-img0003.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # PHB/3229
Concentration
0.2 mg/ml
Product Summary for Prohibitin Antibody (PHB/3229)
Immunogen
Recombinant human Prohibitin protein fragment (aa167-261) (exact sequence is proprietary) (Uniprot: P35232)
Localization
Cytoplasmic (Mitochondria)
Marker
Mitochondrial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08533)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Prohibitin Antibody (PHB/3229)
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602]
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3229).![Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3229-Immunohistochemistry-Paraffin-NBP3-07602-img0002.jpg "Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602]")
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602]
Immunohistochemistry-Paraffin: Prohibitin Antibody (PHB/3229) [NBP3-07602] - Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3229).![Protein Array: Prohibitin Antibody (PHB/3229) [NBP3-07602]](https://resources.bio-techne.com/images/products/Prohibitin-Antibody-PHB-3229-Protein-Array-NBP3-07602-img0001.jpg "Protein Array: Prohibitin Antibody (PHB/3229) [NBP3-07602]")
Protein Array: Prohibitin Antibody (PHB/3229) [NBP3-07602]
Protein Array: Prohibitin Antibody (PHB/3229) [NBP3-07602] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to Prohibitin (PHB/3229).Applications for Prohibitin Antibody (PHB/3229)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Prohibitin
Additional Prohibitin Products
Product Documents for Prohibitin Antibody (PHB/3229)
Product Specific Notices for Prohibitin Antibody (PHB/3229)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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