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Pygopus-1 Antibody (3E1)

Novus Biologicals, part of Bio-Techne | Catalog # H00026108-M13

Novus Biologicals, part of Bio-Techne
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H00026108-M13

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PYGO1 (NP_056432, 101 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YSTFRMPPHVPPRMSSPYCGPYSLRNQPHPFPQNPLGMGFNRPHAFNFGPHDNSSFGNPSYNNALSQNVNMPNQHFRQNPAENFSQIPPQNASQVSNPDL

Specificity

PYGO1 - pygopus homolog 1 (Drosophila) (3E1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Pygopus-1 Antibody (3E1)

Western Blot: Pygopus-1 Antibody (3E1) [H00026108-M13]

Western Blot: Pygopus-1 Antibody (3E1) [H00026108-M13]

Western Blot: Pygopus-1 Antibody (3E1) [H00026108-M13] - Analysis of PYGO1 expression in transfected 293T cell line by PYGO1 monoclonal antibody (M13), clone 3E1. Lane 1: PYGO1 transfected lysate (Predicted MW: 46.09 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Pygopus-1 Antibody (3E1) [H00026108-M13] - Detection limit for recombinant GST tagged PYGO1 is 0.1 ng/ml as a capture antibody.

Applications for Pygopus-1 Antibody (3E1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pygopus-1

PYGO1, also known as Pygopus homolog 1, is a 45kDa 419 amino acid protein. PYGO1 is involved in signal transduction through the Wnt pathway. Current research on PYGO1 is being conducted in relation to acute diarrhea and colorectal cancer. PYGO1 is linked to the WTN signaling pathway where it interacts with BCL9, LENG8, CTNNB1, SPTBN1 and GRB2.

Alternate Names

Pygo1, Pygopus1

Entrez Gene IDs

26108 (Human)

Gene Symbol

PYGO1

Additional Pygopus-1 Products

Product Documents for Pygopus-1 Antibody (3E1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pygopus-1 Antibody (3E1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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