Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Pygopus-1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 300 and 350 of human pygopus homolog 1 using the numbering given in entry NP_056432.1 (GeneID 26108 ).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Pygopus-1 Antibody

Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665]

Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665]

Immunohistochemistry: Pygopus-1 Antibody [NBP1-42665] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Pygo1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665]

Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665]

Immunoprecipitation: Pygopus-1 Antibody [NBP1-42665] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Pygo1 antibody used for IP at 3 mcg/mg lysate. Pygo1 was also immunoprecipitated by rabbit anti-Pygo1 antibody NBP1-42666, which recognizes a downstream epitope.

Applications for Pygopus-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Pygopus-1

PYGO1, also known as Pygopus homolog 1, is a 45kDa 419 amino acid protein. PYGO1 is involved in signal transduction through the Wnt pathway. Current research on PYGO1 is being conducted in relation to acute diarrhea and colorectal cancer. PYGO1 is linked to the WTN signaling pathway where it interacts with BCL9, LENG8, CTNNB1, SPTBN1 and GRB2.

Alternate Names

Pygo1, Pygopus1

Entrez Gene IDs

26108 (Human)

Gene Symbol

PYGO1

Additional Pygopus-1 Products

Product Documents for Pygopus-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pygopus-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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