Raf-1 [p Ser43] Antibody (SR2182)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21574

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21574-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2182

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Raf-1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-Raf-1 [Ser43] (Uniprot #: P04049)

Modification

p Ser43

Specificity

Detects endogenous levels of total phospho-Raf-1 [Ser43]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Raf-1 [p Ser43] Antibody (SR2182)

Western Blot: Raf-1 [p Ser 43] Antibody (SR2182) [NBP3-21574] -

Western Blot: Raf-1 [p Ser 43] Antibody (SR2182) [NBP3-21574] - Western blot analysis using NBP3-21574 in (1) HeLa cell lysate treated with LP; (2) HeLa cell lysate.

Applications for Raf-1 [p Ser43] Antibody (SR2182)

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Raf-1

Raf-1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated Raf-1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the Serine/Threonine specific protein kinases ERK1 and ERK2. Downstream of Raf-1, activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation, and cell migration.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Gene Symbol

RAF1

Additional Raf-1 Products

Product Documents for Raf-1 [p Ser43] Antibody (SR2182)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Raf-1 [p Ser43] Antibody (SR2182)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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