Raf-1 [p Ser642] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-306

Novus Biologicals, part of Bio-Techne
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NB300-306
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Raf-1 Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 conjugated to KLH. Accession # P11365

Modification

p Ser642

Localization

Mitochondrial outer membrane

Specificity

Specific for endogenous levels of the ~74 kDa Raf-1 protein phosphorylated at Ser642. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Raf-1 [p Ser642] Antibody

Western Blot: Raf-1 [p Ser642] Antibody [NB300-306]

Western Blot: Raf-1 [p Ser642] Antibody [NB300-306]

Western Blot: Raf-1 [p Ser642] Antibody [NB300-306] - Analysis of HeLa lysate showing specific labeling of the ~74 kDa Raf 1 protein phosphorylated at Ser642 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.

Applications for Raf-1 [p Ser642] Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This antibody has been tested in Western blot, detecting a band 74 kDa.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Raf-1

The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK. Previous studies have shown that phosphorylation is required for Raf-1 activation. Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf with two sites participating Ser 301 and Ser 642.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Entrez Gene IDs

5894 (Human); 110157 (Mouse); 362281 (Rat)

Gene Symbol

RAF1

UniProt

P11345 (Rat)

Additional Raf-1 Products

Product Documents for Raf-1 [p Ser642] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Raf-1 [p Ser642] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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