Raf-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85462

Novus Biologicals, part of Bio-Techne
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NBP1-85462
NBP1-85462-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Raf-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Raf-1 Antibody

Immunocytochemistry/ Immunofluorescence: Raf-1 Antibody [NBP1-85462]

Immunocytochemistry/ Immunofluorescence: Raf-1 Antibody [NBP1-85462]

Immunocytochemistry/Immunofluorescence: Raf-1 Antibody [NBP1-85462] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody stainig is shown in green.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462]

Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462]

Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human testis shows weak positivity in nuclear speckles in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462]

Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462]

Immunohistochemistry-Paraffin: Raf-1 Antibody [NBP1-85462] - Staining of human cerebellum shows moderate positivity in nuclear speckles in Purkinje cells.
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Applications for Raf-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Raf-1

Raf-1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated Raf-1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the Serine/Threonine specific protein kinases ERK1 and ERK2. Downstream of Raf-1, activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation, and cell migration.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Gene Symbol

RAF1

Additional Raf-1 Products

Product Documents for Raf-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Raf-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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