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Rat CCR9 Alexa Fluor® 700-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8287N

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 882416

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with rat CCR9

Specificity

Detects rat CCR9 in direct ELISAs. Stains rat CCR9 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Rat CCR9 Alexa Fluor® 700-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with rat CCR9 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCR9

CCR9 is an approximately 42 kDa (predicted) seven transmembrane domain receptor for the chemokine CCL25/TECK. It is expressed on CD8+ T cells, monocytes, dendritic cells, and IgA+ plasma cells. It plays a role in oral and gut tolerance, the development of g/d T cells, and the thymic recruitment of hematopoietic progenitor cells. CCR9+ cells limit inflammatory cell infiltration in rheumatoid arthritis, hepatitis, colitis, and inflammatory bowel disease, but exacerbate it in atherosclerosis. In the brain, CCR9 is expressed on CA1 region pyramidal neurons in the hippocampus and is upregulated on microglia during T. gondii infection. CCR9 is also upregulated in a variety of cancers where it promotes tumor cell metastasis. Rat CCR9 shares 86% and 98% amino acid sequence identity with human and mouse CCR9, respectively.

Alternate Names

CCR9, CD199, CDw199

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Gene Symbol

CCR9

Additional CCR9 Products

Product Documents for Rat CCR9 Alexa Fluor® 700-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat CCR9 Alexa Fluor® 700-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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