HEK293 human embryonic kidney cell line transfected with (A) rat CCR9 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CCR9 Monoclonal Antibody (Catalog # MAB8287) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

Applications for Rat CCR9 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with rat CCR9 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is an approximately 42 kDa (predicted) seven transmembrane domain receptor for the chemokine CCL25/TECK. It is expressed on CD8⁺ T cells, monocytes, dendritic cells, and IgA⁺ plasma cells. It plays a role in oral and gut tolerance, the development of g/d T cells, and the thymic recruitment of hematopoietic progenitor cells. CCR9⁺ cells limit inflammatory cell infiltration in rheumatoid arthritis, hepatitis, colitis, and inflammatory bowel disease, but exacerbate it in atherosclerosis. In the brain, CCR9 is expressed on CA1 region pyramidal neurons in the hippocampus and is upregulated on microglia during T. gondii infection. CCR9 is also upregulated in a variety of cancers where it promotes tumor cell metastasis. Rat CCR9 shares 86% and 98% amino acid sequence identity with human and mouse CCR9, respectively.

Alternate Names

CCR9, CD199, CDw199

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Gene Symbol

CCR9

Additional CCR9 Products

Product Documents for Rat CCR9 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat CCR9 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Rat CCR9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8287

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

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Product Summary for Rat CCR9 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat CCR9 Antibody

Detection of CCR9 in HEK293 Human Cell Line Transfected with Rat CCR9 and eGFP by Flow Cytometry.