Rat CD19 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7489
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line recombinant rat CD19
Arg19-Gly287
Accession # NP_001013255
Arg19-Gly287
Accession # NP_001013255
Specificity
Detects rat CD19 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human CD19 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Rat CD19 Antibody
Detection of Rat CD19 by Western Blot.
Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat CD19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7489) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD19 at approximately 85-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Rat CD19 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat splenocytes
Sample: Rat splenocytes
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD19
CD19 (also surface antigen B4 and Leu12) is a 95-110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19-547). Based on mouse, it contains a 269 aa extracellular region (aa 19-287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20-113 and 171-271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19-287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.
Alternate Names
CD19, CVID3, Leu-12
Entrez Gene IDs
Gene Symbol
CD19
UniProt
Additional CD19 Products
Product Documents for Rat CD19 Antibody
Product Specific Notices for Rat CD19 Antibody
For research use only
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