Rat CNTF Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF557

R&D Systems, part of Bio-Techne
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BAF557
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Key Product Details

Species Reactivity

Rat

Applications

ELISA Detection (Matched Antibody Pair), Immunohistochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CNTF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat CNTF
Ala2-Met200
Accession # P20294.1

Specificity

Detects rat CNTF in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross‑reactivity with recombinant human CNTF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat CNTF Biotinylated Antibody

CNTF antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

CNTF in Rat Brain.

CNTF was detected in perfusion fixed frozen sections of rat brain (midbrain) using Goat Anti-Rat CNTF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF557) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat CNTF Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (midbrain) and spinal cord

Western Blot

0.1 µg/mL
Sample: Recombinant Rat CNTF (Catalog # 557-NT)

Rat CNTF Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Rat CNTF Antibody (Catalog # MAB557)
  • Standard: Recombinant Rat CNTF Protein (Catalog # 557-NT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name

Ciliary Neurotrophic Factor

Alternate Names

ciliary neurotrophic factor, HCNTF

Entrez Gene IDs

1270 (Human); 12803 (Mouse); 25707 (Rat)

Gene Symbol

CNTF

UniProt

P20294.1

Additional CNTF Products

Product Documents for Rat CNTF Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat CNTF Biotinylated Antibody

For research use only

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