Rat Cystatin C Antibody

Detection of Rat Cystatin C by Western Blot.

Western blot shows rat plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Cystatin C at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Cystatin C in Rat Brain.

Cystatin C was detected in perfusion fixed frozen sections of rat brain (cerebellum) using Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in the molecular layer of the cerebellum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Rat Cystatin C by Simple Western^TM.

Simple Western lane view shows rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Cystatin C at approximately 28 kDa (as indicated) using 10 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.