Rat VAP-B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7329
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 699811
Product Specifications
Immunogen
E. coli-derived recombinant rat VAP-B
Ala2-Pro132
Accession # Q9Z269
Ala2-Pro132
Accession # Q9Z269
Specificity
Detects rat VAP-B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with
recombinant human (rh) VAP-B and approximately 50% cross-reactivity with
rhVAP-A is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Rat VAP-B Antibody
Detection of Rat VAP‑B by Western Blot.
Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VAP‑B in Rat Brain.
VAP-B was detected in perfusion fixed frozen sections of rat brain (medulla) using Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to gigantocellular neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Rat VAP-B Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (medulla)
Sample: Perfusion fixed frozen sections of rat brain (medulla)
Western Blot
0.5 µg/mL
Sample: PC‑12 rat adrenal pheochromocytoma cell line
Sample: PC‑12 rat adrenal pheochromocytoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VAP-B
Long Name
VAMP [Vesicle-associated Membrane Protein]-associated Protein B and C
Alternate Names
ALS8, VAMP-B, VAMP-C, VAP-C, VAPB
Gene Symbol
VAPB
UniProt
Additional VAP-B Products
Product Documents for Rat VAP-B Antibody
Product Specific Notices for Rat VAP-B Antibody
For research use only
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