Rat/Xenopus Potassium Channel Kv2.2 Antibody

Name: Rat/Xenopus Potassium Channel Kv2.2 Antibody
Brand: R&D Systems
SKU: PPS015

R&D Systems, part of Bio-Techne | Catalog # PPS015

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Product Datasheet / COA / SDS

Voltage Gated

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Rat, Xenopus

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Peptide from the Kv2.2 subunit region of Xenopus and rodent, conjugated to keyhole limpet hemocyanin (KLH)

Specificity

Rodent and Xenopus ~125 kDa Kv2.2 subunit of the Voltage Gated Potassium Channel protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rat/Xenopus Potassium Channel Kv2.2 Antibody

Detection of Potassium Channel Kv2.2 by Western Blot

Western blot of rat brain homogenate. As shown in the autoradiograph, the antibody is specific for the ~125 kDa Voltage Gated Potassium Channel, Kv2.2 subunit. The labeling is blocked by preadsorption with the immunizing peptide.

Applications for Rat/Xenopus Potassium Channel Kv2.2 Antibody

Application

Recommended Usage

Immunohistochemistry

1:1000 dilution
Sample: Frozen sections; unpublished observations

Western Blot

1:1000 dilution
Sample: Rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Potassium Channel Kv2.2

The rat potassium channel Kv2.2 subunit is a 95 - 125 kDa, six transmembrane glycoprotein that belongs to the B subfamily of the potassium channel family. It generates a voltage-dependent channel selective for potassium that helps determine the electrical property of smooth muscle cells, various epithelial cell types and neurons. The symbol K stands for potassium, v for the molecule’s ligand (i.e. voltage), 2 for the family type that is determined by similarity to fruit fly genes, and 2 for the gene number within the family. There are two genes within the Kv2 family (Kv2.1 and Kv2.2) that, although co-expressed, preferentially generate homotetramers rather than heterotetramers. Kv2 family members are associated with action potential repolarization. Kv2.2 is particularly noted for setting the resting membrane potential. Low Kv2.2 activity results in hypersensitive neurons.

References

Trimmer, J.S. and K.J. Rhodes (2004) Annu. Rev. Physiol. 66:477.
Malin, S.A. and J.M. Nerbonne (2002) J. Neurosci. 22:10094.
Blaine, J.T. and A.B. Ribera (1998) J. Neurosci. 18:9585.
Hwang, P.M. et al. (1992) Neuron 8:473.
O’Grady, S.M. and S.Y. Lee (2005) Int. J. Biochem. Cell Biol. 37:1578.

Alternate Names

KCNB2

Entrez Gene IDs

9312 (Human); 98741 (Mouse); 117105 (Rat)

Gene Symbol