Reelin Antibody (RELN/4498)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13874
![Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) [NBP3-13874]](https://resources.bio-techne.com/images/products/Reelin-Antibody-RELN-4498-Immunohistochemistry-Paraffin-NBP3-13874-img0001.jpg "Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) [NBP3-13874]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Mammal
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # RELN/4498
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 1796-2115) of human Reelin protein (Uniprot: P78509)
Reactivity Notes
Rodent
Localization
Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
387.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14203)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Reelin Antibody (RELN/4498)
Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) [NBP3-13874]
Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) [NBP3-13874] - Formalin-fixed, paraffin-embedded human kidneystained with Reelin antibody (RELN/4498). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Reelin Antibody (RELN/4498)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Reelin
Additional Reelin Products
Product Documents for Reelin Antibody (RELN/4498)
Product Specific Notices for Reelin Antibody (RELN/4498)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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