Reelin Antibody (RELN/4498) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14203
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Mammal
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # RELN/4498
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 1796-2115) of human Reelin protein (Uniprot: P78509)
Reactivity Notes
Rodent
Localization
Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
387.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13874).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Applications for Reelin Antibody (RELN/4498) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug per 100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Reelin
Alternate Names
Reeler, RELN, RL
Gene Symbol
RELN
Additional Reelin Products
Product Documents for Reelin Antibody (RELN/4498) - Azide and BSA Free
Product Specific Notices for Reelin Antibody (RELN/4498) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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